Pcr dna sequencing

Pcr is the process  in which. You take any sample of Dna and you take it through these process where you put the Dna sample that you have and places it in a vile. The first step is Pcr amplifications. You add polymers and primers to make many pieces of your desired pieces of Dna. Then by heating and cooling these together in a PTC Machine.  you start making copies of that Dna. With the Polymers and primers that we added. The second step is purification. you put the tubes in a centrifuge to get all the dna separate from the rest. Then the third step is to dilute the product increase volume. Then start process over again. Tell you have as much Dna as you need. IF it is a diesed cell you were looking to find or your babies daddy. You would now have many copies of that cell that you can study if you want to find what the genetic codes to that specific cell. Then you will be able to find what ever you need  to know about that Dna Cell.

Dna Analysis

In this graph it shows who has the higher percents chance of haveing a deseise. the results were graphed from a work sheet that had  the sequences of there dna on it.

Vocab

Anticodon- a triplet of nucleotides in rna designation a specific amino acid that binds to a corresponding codon in rna during protein synthesis

Codon- a sequence of three adjacent nucleotides constituting the genetic code that specifies the insertion of an amino acid

Exon-a nucleic acid sequence that is represented in mature form of an rna molecule either after portions of a precursor

Genetic Code-is a set of rules by which information encoded in genetic material is translated into proteins by living cells.

Intron- are nucleotides sequences within genes that are removed by rna splicing to generate the final mature

mRNA (messenger RNA)-is a molecule of rna encoding a chemical “Blueprint”

Promoter-a regulatory region of dna located up stream of a gene

Protein-coding Gene- transcribes into Mrna

Ribonucleic Acid (RNA)- one of the three major macromolecules that are essential for all known form of life

RNA polymerase-is an enzym that produces Rna.

rRNA (ribosomal RNA)-is the rna component of the ribosome

Transcription- is the process of creating a complementary Rna copy of a sequence of dna

Translation- an act of translating some thing

tRNA (transfer RNA)-is rna that transfers a specific active amino acids

Dna

We experimented with wheat germs to find dna.  Using wheat germ, warm water, detergent and ninety five percent ethanol alcohol. To extract Dna out of the wheat germ. the detergent is what is needed to break the cell wall to release the dna. the dna then floats to the top through the alcohol.